DR CARRIE MADEJ - TRANSGENE HYDRA EXPLAINED

DR CARRIE MADEJ - TRANSGENE HYDRA EXPLAINED

Transgenic Hydra Facility

http://transgenic-hydra.org/

Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis

http://www.pnas.org/content/103/16/6208

Boundary maintenance in the ancestral metazoan Hydra depends on histone acetylation

https://www.researchgate.net/figure/Changes-in-transcriptional-landscape-upon-TSA-treatment-A-C-Generation-of-transgenic_fig3_337296320

Evolutionary crossroads in developmental biology: Cnidaria

https://www.researchgate.net/figure/Transgenic-cnidarians-A-BA-transgenic-colony-of-the-marine-hydrozoan-Hydractinia_fig4_50351101

Doctor: Hydras and Parasites in Vaxx.

 Dr. Ariyana Love joined "The Stew Peters Show",
and made the case that the COV "vaXXines"
contain hydras and parasites,
 and that they're being used to "transfect" humans into a "new species". During the interview,
 Dr. Love begged inoculated people to NOT have children.
Therapeutic Cancer Vaccination with Ex Vivo RNA-Transfected Dendritic Cells—An Update

https://www.researchgate.net/publication/338764293_Therapeutic_Cancer_Vaccination_with_Ex_Vivo_RNA-Transfected_Dendritic_Cells-An_Update

WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate

https://pubmed.ncbi.nlm.nih.gov/26839343/

Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research

https://pubmed.ncbi.nlm.nih.gov/12648161/

Mass Transfer Phenomena in Electroporation

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/electroporation

Nanoparticles versus Dendritic Cells as Vehicles to Deliver mRNA Encoding Multiple Epitopes for Immunotherapy

https://www.researchgate.net/publication/337194074_Nanoparticles_versus_Dendritic_Cells_as_Vehicles_to_Deliver_mRNA_Encoding_Multiple_Epitopes_for_Immunotherapy

Introduction to Transfection | Thermo Fisher Scientific - US

https://www.sciencedirect.com/topics/neuroscience/transfection

mRNA Synthesis and Transfection

https://www.protocols.io/view/mrna-synthesis-and-transfection-9u5h6y6.html

Protocols for Synthetic mRNAs Drive Highly Efficient iPS Cell Differentiation to Dopaminergic Neurons

https://www.protocols.io/view/protocols-for-synthetic-mrnas-drive-highly-efficie-9e5h3g6

Nanoparticulate RNA delivery systems in cancer https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941463/pdf/CNR2-3-e1271.pdf

Hydra Mesoglea Proteome Identifies Thrombospondin as a Conserved Component Active in Head Organizer Restriction

https://www.nature.com/articles/s41598-018-30035-2
High Efficiency mRNA Delivery
https://european-biotechnology.com/needful-things/products/product/high-efficiency-mrna-delivery.html

  Hydra Mesoglea ProteomeIdentifes Thrombospondin as aConserved Component Active inHead Organizer Restriction  

https://www.nature.com/articles/s41598-018-30035-2.pdf

Enlisting the mRNA VaXXine Platform to Combat Parasitic Infections

https://www.researchgate.net/figure/Top-self-amplifying-mRNA-construct-the-sequences-of-four-non-structural-proteins-nsPs_fig1_335954958/download

Biodistribution of in vivo-jetRNA-mRNA complexes via different administration routes
https://www.westburg.eu/products/cell-biology/transfection/in-vivo-transfection/in-vivo-mrna-delivery

Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA-protein correlations at the level of single cells

https://www.researchgate.net/figure/mRNA-labeling-validation-and-transfection-into-cells-using-cationic-lipids-or_fig1_316552213/download

https://www.researchgate.net/publication/316552213_Characterizing_exogenous_mRNA_delivery_trafficking_cytoplasmic_release_and_RNA-protein_correlations_at_the_level_of_single_cells/fulltext/5ae299e3aca272fdaf8fbb4f/Characterizing-exogenous-mRNA-delivery-trafficking-cytoplasmic-release-and-RNA-protein-correlations-at-the-level-of-single-cells.pdf

Experimental attempt to produce mRNA transfected dendritic cells derived from enriched CD34+ blood progenitor cells

https://www.researchgate.net/publication/246274284_Experimental_attempt_to_produce_mRNA_transfected_dendritic_cells_derived_from_enriched_CD34_blood_progenitor_cells/download

HYDRA VULGARIS

Molecular characterization of phospholipid hydroperoxide glutathione peroxidases from Hydra vulgaris

https://pubmed.ncbi.nlm.nih.gov/16919897/

An ancient chordin-like gene in organizer formation of Hydra

https://www.pnas.org/content/104/9/3249

Microinjection to deliver protein, mRNA, and DNA into zygotes of the cnidarian endosymbiosis model Aiptasia sp.

https://www.nature.com/articles/s41598-018-34773-1

Hydra meiosis reveals unexpected conservation of structural synaptonemal complex proteins across metazoans

https://www.pnas.org/content/109/41/16588

Hydra myc2, a unique pre-bilaterian member of the myc gene family, is activated in cell proliferation and gametogenesis

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021362/

Cloning of noggin gene from hydra and analysis of its functional conservation using Xenopus laevis embryos

https://pubmed.ncbi.nlm.nih.gov/20565537/

Deep sequencing reveals unique small RNA repertoire that is regulated during head regeneration in Hydra magnipapillata

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592418/

NANOPARTICULATE DELIVERY SYSTEMSThe ability of NP to therapeutic agents, like siRNA or shRNA for the treatment of genetic disease, such as lung cancer, breast cancer, and Alzheimer's disease makes them a potent tool for gene therapy. 22 , 23 , 24 Their ability to mimic biological membranes and evade the immune system makes them ideal vehicles for drug delivery. The fabrication of a nanoparticulate delivery system, which can be utilized for more specific delivery of drugs has always been a priority for improved disease treatment.
Nanodimensional delivery systems came into existence in the late 1960s, 25 , 26 when researchers exploited the self‐assembling properties of block copolymers 26 and manufactured drug carrying, hollow vehicles. Self‐assembly is a thermodynamically driven and reversible phenomenon, where molecules form a well‐defined structure that is held together by weak, noncovalent forces. Self‐assembling properties of various block copolymers (or surfactants) have been exploited and used successfully to manufacture a biocompatible and biodegradable drug delivering vehicles. 27 , 28 , 29 , 30 Moreover, various shapes of NP can be attained by adjusting the packaging criteria of the self‐assembling molecules (or surfactants), (Figure (Figure1).1). In addition to manufacturing NP, it is also possible to design their shape according to the specific requirements. The purpose of the nanodimensional drug vehicles is to unload the encapsulated drug to the target site, which will further help in the treatment of disease. It is evident that many researchers have manufactured different drug delivery systems and successfully delivered the drug at the point of target, for instance, polyglycerol‐based dendrimers were produced and used by Paula Ofek et al against human glioblastoma and murine mammary adenocarcinoma cells. 31 , 32 , 33 , 34 , 35 The main challenge in drug delivery is to overcome the physiochemical and biological barriers such as the cell membrane, cell environment, blood serum protein, endosomal pathway, and immune system to allow successful drug administration. 36 Moreover, the biodegradable and biocompatible properties are equally important for the development of an effective NP. To achieve a NP associated with all the aforementioned properties, researchers have used different formulations of molecules or surfactants to create NP via various innovative methods.

mRNA-NP Transfection Efficiency in Various Cell Types (A) In vitro transfection efficiency of mRNA-NPs in BMDCs and stromal cell lines (LECs, FRCs, and DAPg7) after 48 h of culture using mCherry-encoding mRNA. The transfection efficiency of BM-DCs was significantly lower than any of the stromal cell lines (p % 0.01). (B-E) In vivo transfection efficiency of mRNA-NPs in different cell types using reporter mRNA in PLNs 48 h after i.p. injection. Data shown in panels B and C are from a study using GFP mRNA and no tissue digestion, while those depicted in panels D and E are from a study using mCherry mRNA and collagenase digestion to retrieve all APC populations. Control mice were untreated mice whose untransfected tissues/cells were used to determine fluorescence background for each cell type. The data are shown as mean of three biological replicates ± SEM (B and D) or as representative plots (C and E). Significant differences in uptake and expression of mRNA indicated for several cell types are relative to LNECs (ECs) for the treated group (D).

original link by PerthObserver

https://www.bitchute.com/video/Abw4HgPrqFBR/