Troubleshooting HPLC
HPLC troubleshooting can be complex, but here are some common issues and potential solutions:
Pressure Problems:
If you're experiencing high pressure, check for clogs in the column or the inlet frit.
Low pressure might be due to a loose connection or a leak. Inspect and tighten fittings.
Baseline Noise:
Air bubbles in the detector flow cell can cause noise. Purge the system to remove bubbles.
Contaminated or degraded mobile phase solvents can also lead to noise. Replace or filter the solvents.
Peak Shape Issues:
Poor peak shape may be caused by a dirty or overloaded column. Try cleaning or replacing it.
Mobile phase pH or composition may not be suitable for your analyte. Adjust accordingly.
Retention Time Drift:
Column temperature fluctuations can affect retention times. Stabilize the temperature.
Inconsistent flow rate can also lead to retention time variability. Check the pump and ensure a steady flow.
No Peaks or Low Sensitivity:
Insufficient sample injection or a weak detector signal can cause this. Optimize injection volume and detector settings.
The column may be saturated or overloaded. Use a lower sample concentration.
Gradient Issues:
Gradient irregularities can occur due to pump problems or mixing issues. Calibrate or service the pump.
Ensure that the gradient delay volume is properly compensated in your method.
System Contamination:
Contaminants in your sample or mobile phase can affect results. Filter the sample and use high-quality solvents.
Detector Problems:
Detector drift or lack of sensitivity may require maintenance or recalibration. Contact the manufacturer for support.
Remember to consult your HPLC instrument manual and seek assistance from technical support or experienced colleagues if troubleshooting becomes challenging
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